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Immunofluorescence analysis of transiently replicating HCV RNAs. A portion of transfected Huh-7 cells (Fig. 3) was seeded onto glass coverslips. NS3 was detected by immunofluorescence at 4 and 96 h posttransfection (left and right sides, respectively). Mock-transfected Huh-7 cells served as a negative control. Bar = 50 μm.
