TABLE 2.
CFU of adapted replicons and copy numbers of HCV RNAs in cell lines obtained after transfection of given replicons and G418 selection
| Replicon | CFU/μg of RNAa | No. of copiesb |
|---|---|---|
| 19 | 72,000 ± 5,000 | 5 ± 1.6 × 107 |
| 5.1 | 510,000 ± 75,000 | 6.9 ± 1.4 × 107 |
| 9-13F | 4,250 ± 650c | 2.6 ± 0.5 × 107 |
| 5B2884Gly | 23,000 ± 3,000c | 5 ± 2.5 × 107 |
a
Mean values and standard deviations derived from 20 to 50 independent transfections of each replicon RNA.
b
Number of HCV replicon RNA molecules per microgram of total RNA as determined by Northern blotting of three independent cell lines of each replicon (Fig. 7). Mean values and standard deviations are from a total of three determinations for each cell line.
c
Data are from the work of Lohmann et al. (29).