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sgRNA control prevents dCas9 toxicity at high inducer concentrations. Growth curves of the XylS/Pm (A/D), LacI/Plac (B/E), and AraC/PBAD (C/F) expression systems in 0 and high levels of inducer. Blue = KT2440 + dCas9, red = KT2440 + dCas9 + pSpyB-empty, and green = KT2440 + dCas9 + pSpyB-Tn7sg. Data are the mean ± standard error of 3 replications.
