FIG. 5.
Analysis of SFV structural protein synthesis, processing, and release. Total NP-40 lysates from cells transfected with SFV-WT, SFV-M113A, SFV-I115A, or SFV-M113A/I115A were run on SDS–10% PAGE. Cells were labeled with [35S]methionine 7 h posttransfection and chased for 10 min or 2 h. Medium from the longer chase time was loaded on top of a 20% sucrose cushion and centrifuged. The pelleted material was solubilized directly in loading buffer. Lanes 1 to 3, SFV (10-min chase, 2-h chase, and pellet); lanes 4 to 6, SFV-M113A; lanes 7 to 9, SFV-I115A; lanes 10 to 12, SFV-M113A/I115A.