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. Author manuscript; available in PMC: 2024 Sep 24.
Published in final edited form as: J Vis Exp. 2021 Sep 24;(175):10.3791/63045. doi: 10.3791/63045

Table 2:

Extracellular flux analyzer program for the glycolysis stress test.

1 Calibration
2 Equilibration
3 Injections and measurements
Cycles Mix Wait Measure
Baseline (Non-glycolytic acidification) 3 times 3 min 0 min 3 min
Inject Port A: Glucose 3 times 3 min 0 min 3 min
Inject Port B: Oligomycin 3 times 3 min 0 min 3 min
Inject Port C: 2-DG 3 times 3 min 0 min 3 min

Abbreviation: 2-DG = 2-deoxy-D-glucose.