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Western blot analysis for surfactant proteins A and D [SP-A (1A) and SP-D (1B)] after 1-dimensional electrophoresis under reducing conditions in which equine synovial fluid (SF) proteins were compared with partially purified surfactant from equine bronchoalveolar lavage fluid as a positive control. Immunodetection was done with anti-SP antibodies, an exposure time of 1 min, and enhanced chemiluminescence.
