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. 2001 May;75(10):4633–4640. doi: 10.1128/JVI.75.10.4633-4640.2001

FIG. 1.

FIG. 1

Schematic representation of the KUN plasmid DNA constructs (A) and of the complementation experiments (B) conducted with the normal BHK cells and with the helper BHK cells (repBHK) stably expressing the KUN replicon RNA. Filled boxes show the translated region of the KUN genome with numbers representing amino acid positions (A) and the KUN replicon sequence (B). Also shown are the 5′ and 3′ KUN UTRs, the cytomegalovirus early promoter-enhancer region (CMV promoter), and the antigenomic sequence of the hepatitis delta virus ribozyme (HDVr). The HDVr sequence ensures generation of the correct KUN 3′ terminus. SV40 poly(A) in panel A and the asterisk in panel B show the simian virus 40 polyadenylation signal; the filled oval (B) indicates a poly(A) sequence. dGDD refers to the deletion of the RNA polymerase motif GDD in the NS5 gene, shown in bold and underlined letters in pKUN1 and as dashes in pKUN1dGDD (A), and the filled circle in front of the KUN RNA (B) represents the cap structure. For the explanation of the experimental design shown (B), see the text.