Table I.
Flow cytometric analysis of isolated peripheral blood mononuclear cells
| Receptor | Treatment | D0 | D14 | D28 | C7 | C14 | C21 |
|---|---|---|---|---|---|---|---|
| CD2 | FICA | 29.3 ± 5.7 | 27.2 ± 4.0ab | 29.4 ± 4.1 | 29.4 ± 10.4 | 25.9 ± 3.9 | 27.6 ± 6.7 |
| T | 30.5 ± 5.2 | 23.6 ± 4.0b | 30.9 ± 4.9 | 23.2 ± 3.9 | 27.5 ± 4.0 | 23.4 ± 2.6 | |
| T + F | 34.7 ± 5.2 | 37.0 ± 3.5a | 36.0 ± 4.2 | 33.3 ± 5.7 | 34.6 ± 5.7 | 30.5 ± 2.7 | |
| T + A | 36.9 ± 5.2 | 38.6 ± 4.7a | 35.4 ± 4.2 | 32.1 ± 6.0 | 30.5 ± 5.7 | 36.6 ± 4.8 | |
| CD3 | FICA | 38.5 ± 5.5 | 37.2 ± 3.5 | 36.7 ± 6.0 | 39.2 ± 10.5 | 38.6 ± 13.0 | 35.6 ± 6.4 |
| T | 38.9 ± 5.5 | 30.9 ± 3.6 | 36.9 ± 3.2 | 32.4 ± 3.5 | 34.8 ± 6.0 | 31.1 ± 4.6 | |
| T + F | 42.6 ± 7.5 | 36.2 ± 2.4 | 42.2 ± 4.1 | 41.6 ± 3.7 | 36.6 ± 5.1 | 35.7 ± 3.0 | |
| T + A | 43.5 ± 3.7 | 42.0 ± 4.1 | 43.6 ± 6.4 | 36.5 ± 5.3 | 36.0 ± 4.0 | 42.5 ± 7.8 | |
| CD4 | FICA | 10.7 ± 3.9 | 15.1 ± 1.0 | 8.6 ± 3.0b | 8.7 ± 5.6 | 11.2 ± 5.3 | 15.4 ± 6.8 |
| T | 15.3 ± 3.9 | 15.6 ± 2.1 | 20.4 ± 3.3a | 12.4 ± 2.5 | 16.7 ± 1.9 | 15.4 ± 1.7 | |
| T + F | 20.1 ± 4.8 | 16.6 ± 1.2 | 17.7 ± 0.2ab | 18.2 ± 1.2 | 18.0 ± 3.8 | 11.6 ± 3.0 | |
| T + A | 11.9 ± 5.3 | 20.4 ± 6.0 | 20.4 ± 2.7a | 18.8 ± 3.7 | 12.6 ± 4.0 | 15.6 ± 7.3 | |
| CD8 | FICA | 8.3 ± 2.6 | 8.9 ± 2.3ab | 8.1 ± 0.6 | 4.0 ± 2.7 | 7.6 ± 1.3ab | 8.2 ± 2.3 |
| T | 7.5 ± 1.0 | 5.6 ± 0.5b | 7.9 ± 1.0 | 5.6 ± 0.8 | 5.7 ± 0.9b | 8.0 ± 1.7 | |
| T + F | 12.3 ± 3.5 | 11.2 ± 1.6a | 2.5 ± 4.4 | 10.4 ± 3.5 | 12.2 ± 1.6a | 7.0 ± 2.0 | |
| T + A | 8.3 ± 1.8 | 12.1 ± 2.3a | 9.9 ± 1.4 | 10.9 ± 3.2 | 12.0 ± 2.4ab | 15.5 ± 3.1 | |
| MHCII | FICA | 47.0 ± 9.6 | 56.2 ± 6.5a | 47.1 ± 12.9 | 37.2 ± 12.1 | 52.3 ± 9.3 | 58.4 ± 12.4 |
| T | 36.4 ± 9.2 | 48.3 ± 7.2a | 37.3 ± 9.5 | 44.5 ± 8.6 | 50.6 ± 9.9 | 59.1 ± 8.7 | |
| T + F | 27.1 ± 5.5 | 27.1 ± 4.9b | 36.6 ± 4.3 | 36.7 ± 8.3 | 38.7 ± 6.0 | 37.0 ± 3.2 | |
| T + A | 37.6 ± 8.7 | 37.9 ± 6.4ab | 34.0 ± 8.4 | 47.6 ± 6.4 | 42.9 ± 11.1 | 37.4 ± 5.0 | |
| B cell | FICA | 30.7 ± 8.4 | 38.0 ± 13.1 | 40.9 ± 10.3 | 25.8 ± 8.0 | 31.4 ± 7.5 | 45.0 ± 10.3 |
| T | 28.7 ± 9.8 | 35.4 ± 8.9 | 27.4 ± 7.7 | 30.8 ± 7.4 | 39.5 ± 10.7 | 51.1 ± 12.7 | |
| T + F | 22.0 ± 5.7 | 24.7 ± 5.3 | 23.8 ± 3.4 | 24.0 ± 2.6 | 31.8 ± 3.0 | 26.6 ± 4.7 | |
| T + A | 24.2 ± 5.4 | 26.6 ± 4.5 | 26.0 ± 5.1 | 31.8 ± 6.4 | 30.2 ± 6.0 | 25.8 ± 3.2 |
Values are mean ± sχ̄ of the percentage of positive-stained cells and are compared with other treatments at each time point
ab
Values with different superscripts are significantly different at P < 0.05
CD — ; FICA — Freud’s incomplete adjuvant; T — trivalent only; T + F — trivalent and Freud’s incomplete adjuvant; T + A — trivalent in aluminum hydroxide; MHC — major histocompatibility complex