FIGURE 6—

Using the rotator, the volume of a three-dimensional object can be estimated in two-dimensional tissue sections. For each neuron identified within the sampling frame, the neuronal nuclear volume was estimated. Measurements were pooled within a group and binned into 20 size ranges. The percentage of nuclear volume measurements within each bin size is plotted for each group at 10–15 min after sham or brain injury. After mild and moderate brain injury, the nuclear size distribution shows a shift toward smaller volumes compared with the sham group. To provide a reference point, the mean nuclear volume for the sham group is indicated by the black vertical line in each graph.