Fig. 5.

DSP knockdown rescues the migration and invasion deficits caused by DAPK3 knockout in SUM159 cells. A) Immunoblotting shows the protein levels of DAPK3, DSP, and JUP in SUM159 control cells, DAPK3 KO #3 cells, and DAPK3 KO #3 cells with DSP knockdown mediated by two different shRNAs. GAPDH serves as a loading control. The representative image is based on three independent experiments. B) Representative transwell migration and invasion assay images (10× magnification) from SUM159 control cells, DAPK3 KO #3 cells, and DAPK3 KO #3 cells with DSP knockdown. DSP knockdown by two different shRNAs increases migration and invasion potential compared with nonsilencing shRNA in DAPK3 KO #3 cells. C), D) ImageJ-based quantification results of B. Data represent the mean ± SEM from three independent experiments. ****P < 0.0001, ***P < 0.001, **P < 0.01, one-way ANOVA with Tukey's multiple comparisons test.