Table 1b.

Intensity of crystal spherulites in trout kidney wet mount sections.

DoseMEL + CYA (mg/kg bw each)	Estimated equivalent in feed (ppm)	# Daily doses	Initial phase (intensity)	Confirmatory phase (intensity)	Comment
20	500	1	0,0,0,1,2,2		(results from depletion study)
10	250	1	0,0,0	0,0,1,2,2,2,2,2,3
5	125	1	0,0,0,0,0,3
2.5	62.5	1	0,0,0,0,0,0	0,0,0,0,0,0	NOAEL
5	125	4	1,2,2,3,0,0		These were done concurrently w/single dose
2.5	62.5	4	0,0,0,0,0,0		Starting test dose for 14 day study
2.5	62.5	14	4,4,4,3,3,3
1	25	14	3,2,1,1,0,0
0.5	12.5	14	0,0,0,0,0,0	0,0,0,0,0,0	NOAEL

The intensity of melamine–cyanurate crystal spherulites seen on wet mount sections of kidney. Each intensity value represents the finding in an individual fish, initial phase n = 3–6, confirmatory phase n = 3–9.

Intensity Scale: 1 – only 1 in an entire section; 2 – few with scattered distribution; 3 – moderate numbers seen throughout section; 4 – large numbers seen immediately; and 5 – extensive numbers obliterating the regular tissue architecture.

Estimated equivalent in feed corresponds to the mg/kg bw dose if fish are fed 4% of their body weight per day.