Figure 5.

Gelatinase release from blood and milk neutrophils of normal lactating goats. Neutrophil samples were incubated with and without 1 ng/mL PMA at 37°C for 1 h. The supernatants were collected by centrifugation and subjected to gelatin-containing agarose gel electrophoresis. (A) Gelatinase activity detected on the gelatin zymogram for 3 representative goats. (B) Relative gelatinase activity quantified by densitometrically scanning the photographic negative of zymogram for blood (▪) and milk (□) neutrophils. Values represent means ± sχ̄. Comparisons were made between blood and milk neutrophils under the same conditions. a(P < 0.05) b(P < 0.01).