Table 7.
Crystal rankings and melamine and cyanuric acid concentrations in serum and amniotic fluid of pregnant female rats administered melamine or cyanuric acid for 10 consecutive days at a dosage of 1000 mg/kg BW/day. Values on one line correspond to the same rat. Serum from all pregnant control rats (n = 11) was negative for both compounds.
| Dose Group | Plate-like clear crystals | CYA (μg/mL) | MEL (μg/mL) | ||
|---|---|---|---|---|---|
| Serum | Amniotic Fluid | Serum | Amniotic Fluid | ||
| Cyanuric Acid | 3 | 313.7 | 123.5 | <LOD | <LOD |
| 2 | 152.5 | 157.4 | <LOD | <LOD | |
| 0 | 80.5 | 60.3 | <LOD | <LOD | |
| 0 | 55.8 | 51.8 | <LOD | <LOD | |
| 0 | 41.6 | 40.4 | <LOD | <LOD | |
| 0 | 31.1 | 44.5 | <LOD | <LOD | |
| 0 | 23.0 | 33.9 | <LOD | <LOD | |
| 0 | 16.7 | 37.7 | <LOD | <LOD | |
| 0 | 15.3 | 28.3 | <LOD | <LOD | |
| 0 | 11.3 | 37.4 | <LOD | <LOD | |
| 0 | 10.8 | 23.9 | <LOD | <LOD | |
| Mean | 68.4 | 58.1 | 0.0 | 0.0 | |
| SD | 91.5 | 42.6 | 0.0 | 0.0 | |
| Melamine | 5 | <LOD | <LOD | 683.2a | 472.5a |
| 5 | <LOD | <LOD | 610.9 | 497.1 | |
| 5 | <LOD | NS | 551.8 | NS | |
| 4 | <LOD | <LOD | 411.3b | 517.2b | |
| 5 | <LOD | <LOD | 409.0 | 377.0 | |
| 4 | <LOD | <LOD | 355.6 | 300.6 | |
| 3 | <LOD | NS | 294.1c | NSc | |
| 4 | <LOD | NS | 116.9 | NS | |
| 5 | <LOD | <LOD | 89.6 | 346.4 | |
| 5 | NS | NS | NSd | NSd | |
| 5 | NS | NS | NSe | NSe | |
| 5 | NS | NS | NSe | NSe | |
| 4 | NS | NS | NSd | NSd | |
| Mean | 0.0 | 0.0 | 391.4 | 418.5 | |
| SD | 0.0 | 0.0 | 205.2 | 89.1 | |
LOD = limit of detection (0.4 μg/mL). NS = no sample (amniotic fluid could not be collected from some rats for reasons discussed in the text).
a,b
Removed early from study at GD15 and 17, respectively.
c,d,e
Found dead at GD18, 14, and 16, respectively.