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. 2024 Sep 24;19(9):e0308821. doi: 10.1371/journal.pone.0308821

Fig 1. The PrPC lowering potency of KDC203 affects all post-translational PrPC isoforms but is reduced in rich culture media.

Fig 1

(A) KDC203 concentration-dependent reduction of all PrPC isoforms is apparent in PNGase F treated human T98G cell extracts. The Coomassie stain documents equal loading. (B) Human LN-229 cells exhibit a KDC203 concentration-dependent increase in total PrPC levels. (C) The capacity of KDC203 to lower PrPC levels in T98G cells is reduced in serum-rich media. (D) The KDC203-dependent increase of PrPC levels in LN-229 levels is reduced in serum-rich media. (E) The cell surface pool of PrPC that can be released into the supernatant by PI-PLC digestion dominates total PrPC levels in T98G cells and is strongly reduced upon KDC203 treatment. ATP1A1 levels are also reduced in KDC203-treated T98G cells but cannot be observed in the PI-PLC digestion supernatant. (F) KDC203 treatment caused an increase in total ATP1A1 and PrPC levels in LN-229 cells yet reduced the PI-PLC-releasable cell surface pool of PrPC.