Table I.
Virus detection by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunofluorescence assay (IFA) in various organs and serological response detected by enzyme-linked immunosorbent assay (ELISA) in serum samples from specific pathogen free (SPF) chickens inoculated with immune complex infectious bursal disease vaccine (IBDV-Icx)
| RT-PCR
|
IFA | ELISA | ||||||
|---|---|---|---|---|---|---|---|---|
| Hours and days PV | BF | SP | TH | CT | L | D | BF | Serum |
| 5h | 0/1a | 0/2 | 0/3 | nd | 0/3 | 0/3 | 0/1 | 1.78b |
| 1 | 0/3 | 0/2 | 0/3 | nd | 0/3 | 0/3 | 0/1 | 2.88 |
| 3 | 0/3 | 0/3 | 0/3 | nd | 0/3 | 0/2 | 0/1 | 2.82 |
| 6 | 0/3 | 0/3 | 0/3 | 0/1 | nd | nd | 0/1 | 2.09 |
| 10 | 0/3 | 0/3 | 0/3 | 0/1 | 0/3 | 0/3 | 0/1 | 3.24 |
| 14 | 3/3 | 0/2 | 0/3 | 0/2 | nd | nd | 1/1 | 832.00 |
| 21 | 3/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/2 | 1/1 | 794.00 |
nd — Not determined; PV — post vaccination; BF — bursa of Fabricius; SP — spleen; TH — thymus;
CT — cecal tonsils; L — liver; D — duodenum
a
Number positive/total tested
b
Results are expressed as geometric mean antibody titers (GMT) of 3 serum samples