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. 2005 Mar 23;2(2):191–199. doi: 10.1093/ecam/neh077

Figure 4.

Figure 4

Application of Juzen-taiho-to, Hochu-ekki-to, Shofu-san, Oren-gedoku-to and prednisolone influence the expression of IL-4 and IFN-γ mRNA in the cervical lymph nodes and ears of NC/Nga mice treated repeatedly with mite antigen. Cervical lymph nodes and ears were obtained 4 h after the fifth antigen application. Expression of mRNA was examined using RT–PCR. Resultant electrophoretic bands were semi-quantitatively evaluated using NIH Image software. Experiments were repeated at least twice and a representative experiment is indicated. Results are expressed as a ratio of (A) IL-4 and (B) IFN-γ to β-actin mRNA expression. Non, no antigen; Cont, control; TJ-48, Juzen-taiho-to; TJ-41, Hochu-ekki-to; TJ-22, Shofu-san; TJ-15, Oren-gedoku-to; Pred, prednisolone; ND, not detected.