FIG. 4.

Effect of NO donors on replication in infected T-cell lines. Jurkat and MT-2 cell lines infected with HIV-1 are shown. SNAP (100 μM) and SNP (100 or 300 μM) were added to the cultures as indicated. HIV-1 p24 Ag content in the supernatants was determined in triplicate cultures by an Ag capture immunoassay at day 3 after infection. Data shown are the means ± SD of triplicate cultures from three independent experiments. The differences are statistically significant (P < 0.01) for 100 μM SNAP, 100 μM SNP, and 300 μM SNP in Jurkat cells and for 100 μM SNP (P < 0.05) and 300 μM SNP (P < 0.01) in MT-2 cells with respect to the HIV-1 controls.