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Activation of the TNF-α promoter by HIV-1 infection in Jurkat and MT-2 cells. Cell lines were transfected with the reporter plasmid TNF-α-luc. After transfection, cells were infected with HIV-1 (MOI, 0.5) (HIV-1 0.5) or treated with heat inactivated HIV-1 (MOI, 50) (hi HIV-1 50). Luciferase activity in relative light units (RLU) was determined 16 h later. Data shown are the means ± SD of two independent experiments.
