FIG. 9.

Quantification of surface DC-SIGN required for virus transmission. 293 T-Rex DC-SIGN cells were seeded in duplicate and incubated overnight with increasing concentrations of doxycycline. One panel was used in the virus transmission assay as described in Materials and Methods; the other panel was used for quantification of surface DC-SIGN expression via QFACS, employing the anti-AU1 antibody. (A) DC-SIGN expression upon induction with doxycycline. Overlay histogram shows DC-SIGN staining with the anti-AU1 antibody upon induction with, doxycycline at 0.01 (black) and 0.0001 (light grey) μg/ml compared to control staining with mouse (dark grey) immunoglobulin G and no antibody (white). (B) Virus transmission to C8166 T cells. Virus transmission efficiency was normalized to optimal transmission and is shown relative to the number of ABS. Data from one representative experiment carried out in triplicate are shown. Comparable results were obtained in two independent experiments.