Table 3.
Prediction of B-cell epitopes on the lpqH, MTP and PstS1 adhesin proteins using various immunoinformatic tools.
| B Cell Epitope Prediction Tool | Predicted Epitope Sequence for each Protein and Score (if available) |
|||
|---|---|---|---|---|
| tnHSP70 | lpqH | MTP | PstS1 | |
| BepiPred | EVKDVLLLDVTPLSLGIETKGGVMTRLIERNTTIPTKRSETFTTADDNQPSVQIQVYQGEREIAAHNKLLGSF | SSNKSTTGSGETTTAAGTTASPGAASG | AAQTAPVPDY | GASDAYLSEGDMAAHK |
| GKDQNVT | AWGPNWDPYTCHDDFHRDSDGPDHSRDYPGP | MNIALAISAQQVNYNLPGVSEHLKLNGKVLAA | ||
| DGNPPEVKSVGLGNVN | GPVLDDPGAAPPPPAAG | IKTWDDPQIAALNPGVNL | ||
| QIEVTFDIDANGIVHVTAKDKGTGKENTIRIQEGSGLSKEDIDRMIKDAEAHAEEDRKRREEADVRNQAETLVYQ | YTSGTGQGNASATK | SKQDPEGWGKSPGFGTTVDFPAVPGALGENGNGG | ||
| GVDMANPMSPVNKS | QASQRGL | |||
| SSGNFLLP | ||||
| KEQREAEGGSKVPEDTLNKVDAAVAEAKAALGGSDISAIKSAMEKLGQESQALGQAIYEAAQAASQATGAAHPGGEPGGAHP | FASKTPANQAISMIDGPAPD | |||
| NRQKDAATAQ | ||||
| SFLD | ||||
| HFQP | ||||
| ABCpred | EAHAEEDRKRREEADV (0.91) | VTGSVVCTTAAGNVNAIGGA (0.83) | YYWCPGQPFDPAWGP (0.94) | DFPAVPGALGENGNGGMVTG (0.86) |
| RGIPQIEVTFDIDANG (0.90) | TTASPGAASGPKVVIDGKDQ (0.82) | QSAAQTAPVPDYYWCP (0.93) | LHRSDGSGDTFLFTQYLSKQ (0.85) | |
| TKRSETFTTADDNQPS (0.86) | TGIAAVLTDGNPPEVKSVGL (0.81) | HRDSDGPDHSRDYPGP (0.91) | LSEGDMAAHKGLMNIALAIS (0.82) | |
| AHPGGEPGGAHPGSAD(0.86) | GSGETTTAAGTTASPGAASG (0.80) | GPILEGPVLDDPGAAP (0.87) | IINYEYAIVNNRQKDAATAQ (0.81) | |
| KGGVMTRLIERNTTIP (0.86) | KVVIDGKDQNVTGSVVCTTA(0.80) | AACILATGVAGLGVGA (0.85) | LGNSSGNFLLPDAQSIQAAA (0.81) | |
| DRMIKDAEAHAEEDRK (0.86) | TLGYTSGTGQGNASATKDGS (0.79) | GPNWDPYTCHDDFHRD (0.83) | YIGISFLDQASQRGLGEAQL (0.81) | |
| GLSKEDIDRMIKDAE (0.83) | TGQGNASATKDGSHYKITGT (0,76) | DDPGAAPPPPAAGGGA (0.82) | NGNGGMVTGCAETPGCVAYI (0.81) | |
| KVPEDTLNKVDAAVAE (0.82) | AAGNVNAIGGAATGIAAVLT (0,74) | PDHSRDYPGPILEGPV (0.82) | EHLKLNGKVLAAMYQGTIKT (0.80) | |
| GNPPEVKSVGLGNVNGVTLG (0.55) | TGVAGLGVGAQSAAQT (0.73) | GCAETPGCVAYIGISFLDQA (0.79) | ||
| ABCpred | FELTGIPPAPRGIPQI (0.80) | RFACRTLMLAACILAT (0.54) | QIAALNPGVNLPGTAVVPLH (0.78) | |
| EAEGGSKVPEDTLNKV (0.80) | NYNLPGVSEHLKLNGKVLAA (0.78) | |||
| IERNTTIPTKRSETFT (0.79) | LHWAITDGNKASFLDQVHFQ (0.78) | |||
| GQAIYEAAQAASQATG (0.79) | AGFASKTPANQAISMIDGPA (0.77) | |||
| GQESQALGQAIYEAAQ (0.78) | LPGTAVVPLHRSDGSGDTFL (0.76) | |||
| SLGIETKGGVMTRLIE (0.78) | GTIKTWDDPQIAALNPGVNL (0.76) | |||
| TFDIDANGIVHVTAKD (0.77) | FLFTQYLSKQDPEGWGKSPG (0.74) | |||
| HVTAKDKGTGKENTIR (0.77) | VNNRQKDAATAQTLQAFLHW (0.72) | |||
| QAASQATGAAHPGGEP (0.75) | PDAQSIQAAAAGFASKTPAN (0.72) | |||
| GSDISAIKSAMEKLGQ (0.74) | QASQRGLGEAQLGNSSGNFL (0.72) | |||
| RKRREEADVRNQAETL (0.71) | DGPAPDGYPIINYEYAIVNN (0.68) | |||
| SVQIQVYQGEREIAAH (0.69) | LMNIALAISAQQVNYNLPGV (0.67) | |||
| SAMEKLGQESQALGQA (0.68) | QDPEGWGKSPGFGTTVDFPA (0.66) | |||
| FTTADDNQPSVQIQVY (0.67) | KVLAAMYQGTIKTWDDPQIA (0.61) | |||
| ETLVYQTEKFVKEQRE (0.65) | ||||
| YQGEREIAAHNKLLGS (0.63) | ||||
| SVM TRiP | HAEEDRKRREEADVRNQAET (1.000) | SGETTTAAGTTASPGAASGP (1.00) | DMAAHKGLMNIALAISAQQV (1.000) | |
| DISAIKSAMEKLGQESQALG (0.563) | DGKDQNVTGSVVCTTAAGNV (0.998) | PLHRSDGSGDTFLFTQYLSK (0.998) | ||
| IAAHNKLLGSFELTGIPPAP (0.435) | DGNPPEVKSVGLGNVNGVTL (0.997) | SQRGLGEAQLGNSSGNFLLP (0.990) | ||
| AEGGSKVPEDTLNKVDAAVA (0.353) | LPGVSEHLKLNGKVLAAMYQ (0.351) | |||
| DIDANGIVHVTAKDKGTGKE (0.353) | QAAAAGFASKTPANQAISMI (0.294) | |||
| QAIYEAAQAASQATGAAHPG (0.203) | ||||