Table 5.
Physicochemical and protein parameters of trifusion constructs identified using the ProtParam ExPasy Tool
| Protein Parameters | tnLPH | tnMPH |
|---|---|---|
| Number of amino acids | 636 | 617 |
| Molecular weight | 64865.80 | 63958.62 |
| Theoretical pI | 4.77 | 4.63 |
| Total number of negatively charged residues (Asp + Glu) | 73 | 79 |
| Total number of positively charged residues (Asp + Glu) | 45 | 43 |
| Formula | C2809H4458N798O942S12 | C2793H4336N782O917S13 |
| Total number of atoms | 9019 | 8841 |
| Extinction coefficients all pairs of Cys residues form cystines all Cys residues are reduced | 35995 | 55600 |
| 35870 | 55350 | |
| Estimated half-life | 30 h (mammalian reticulocytes, in vitro | 1 h (mammalian reticulocytes, in vitro |
| >20 h (yeast, in vivo) | 30 min (yeast, in vivo) | |
| >10 h. (E. coli, in vivo) | >10 h. (E. coli, in vivo) | |
| Instability Index | 27.52 | 34.23 |
| Aliphatic index | 76.79 | 70.97 |
| Grand average of hydropathicity (GRAVY) | −0.29 | −0.406 |