Fig. 2. Optimal blood glucose homeostasis in mice whose islets comprise β-cells only.
a, Glycaemia over 40 wpa in Ctrl (n = 6) and DT (n = 6) mice under random-fed (RF) or fasting (F) conditions. Right panel: area under the curve (AUC). P values (DT vs Ctrl): RF, P = 0.73; F, P = 0.3939. b, Intraperitoneal glucose tolerance test (ipGTT) on Ctrl (n = 33) and DT (n = 34). Right panel: AUC P value (Ctrl vs DT), P = 0.3. c, Plasma insulin 0 min and 15 min after glucose injection along ipGTT in Ctrl (n = 25) and DT (n = 25) mice. Right panel: stimulation index of insulin secretion. P value (Ctrl vs DT), **P = 0.0074. d, Oral glucose tolerance test (oGTT) on Ctrl (n = 7) and DT (n = 11). Right panel: AUC P value (Ctrl vs DT), **P = 0.0028. e, Plasma GLP-1 along oGTT in Ctrl (n = 5) and DT (n = 7) mice. P value (Ctrl vs DT), *P = 0.0303. f, Plasma insulin 0 min and 10 min after glucose gavage along oGTT in Ctrl (n = 8) and DT (n = 11) mice. Right panel: stimulation index; P value (Ctrl vs DT), ***P = 0.0006. g, Insulin tolerance test (ITT) on Ctrl (n = 6) and DT (n = 6) mice. Data as % relative to glycaemia at time 0. Right panel: AUC of ITT; P value Ctrl vs DT, *P = 0.03. h, Radiolabelled 2-deoxy-d-glucose (2DG) uptake by WAT in Ctrl (n = 6) and DT (n = 6) mice. P values (Ctrl vs DT), *P = 0.039. i, Energy expenditure (EE) in Ctrl (n = 9) and DT (n = 7) mice. Right panel: AUC of EE, P value: light period Ctrl vs DT, **P = 0.0012; Ctrl dark vs light period, P < 0.0001; DT dark vs light period, **P = 0.0047. j, Respiratory exchange ratio (RER) in Ctrl (black, n = 9) and DT (red, n = 7) mice. Right panel: AUC of RER. P value: light period Ctrl vs DT, *P = 0.035; Ctrl dark vs light period, P < 0.0003; DT dark vs light period, P = 0.1212. Only male mice were used. All data are shown as mean ± s.e.m.; P values are from two-tailed Mann–Whitney tests.