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. 2024 Aug 21;6(9):1791–1806. doi: 10.1038/s42255-024-01114-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Extended Data Fig. 2 — a, Body weight over 40weeks on Ctrl (black, n = 6) and DT (red, n = 6) mice in random fed (RF, continuous line) and fasting (F, dashed line). Right panel: Area under the curve (AUC). P-values (DTvsCtrl): RF P = 0.3312, F P = 0.3939. b, Body composition in Ctrl (black, n = 8) and DT (red, n = 8) mice. P-values CtrlvsDT: Lean mass P = 0.7209, Fat mass P = 0.083. c, Immunofluorescence on SST (red) in stomach (upper micrographs) and GLP-1 (red) in colon (lower micrographs) from Ctrl or β-only mice. Scale bar: 20 µm or 10 µm (insets). d, Duodenum qPCR of Sst of Ctrl (black, n = 4) and β-only mice at 7 dpa (pink, n = 7), 15 dpa (light red, n = 7), and 30 dpa (red, n = 6). Data are normalized ct values relative to β-actin and Gapdh and shown as relative hormone expression to Ctrl. P-values: 7 dpa vs Ctrl P = 0.0061, 15 dpa vs Ctrl P = 0.0606, 30 dpa vs Ctrl P = > 0.999. e, Plasma somatostatin in Ctrl (n = 16) and β-only after 15 dpa (pink, n = 8) and 30 dpa (red, n = 8). P-values: 15 dpa vs Ctrl P = 0.0016, 30 dpa vs Ctrl P = 0.1090, 30 dpa vs 15 dpa P = 0.0468. f, Brain qPCR on Sst of Ctrl (black, n = 4) and β-only (red, n = 6). Data are normalized ct values relative to β-actin and Gapdh and shown as relative hormone expression to Ctrl. P-values Ctrl vs DT: P = 0.0095. g, Brain somatostatin of Ctrl (n = 8) and β-only (n = 7). P-values: DT vs Ctrl P = 0.0128. h-j, Food intake (h; gr/gr), total movement (i; counts, infrared breaks in 15 minutes) and running wheel activity (j) in Ctrl (black, n = 9) and DT (red, n = 7). Right panel: AUC. Food intake (h) P-value (CtrlvsDT): light period P = 0.2523; dark period P = 0.6065; 24 h P = 0.4079. Total movement (i) P-value (CtrlvsDT): light period P = 0.2105; dark period P = 0.8371; 24 h period P = 0.7577. Running wheel (j) P-value (CtrlvsDT): light period P = > 0.99; dark period P = 0.9182; 24 h period P = > 0.99. Only male mice were used. All data are shown as mean ± sem. All statistical tests are two-tailed Mann-Whitney test. Source data is available.

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