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. 2024 Sep 9;165(10):bqae116. doi: 10.1210/endocr/bqae116

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© The Author(s) 2024. Published by Oxford University Press on behalf of the Endocrine Society.

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Figure 4. — Normal puberty onset and gonad weights in gonadotrope-specific Zeb1 knockout mice. (A) Genomic DNA was extracted from the indicated tissues of Zeb1^fl/fl (control; gray) and Zeb1^fl/fl; Gnrhr^GRIC/+ (cKO; red) mice and analyzed by PCR for the presence of the floxed or recombined (rec) Zeb1 alleles. (B) Pituitary sections from control and cKO mice were analyzed for ZEB1 by immunofluorescence (blue); LHβ (red) was used to label gonadotropes. Scale bars: 20 μm. (C) Age of vaginal opening (days) in female control and cKO mice. (D) Age of preputial separation (days) in male control and cKO mice. (E) Proportion of time spent in each stage of the estrous cycle (over 21 days) in female control and cKO females; metestrus (M)/diestrus (D), proestrus (P), estrus (E). (F) Ovarian weights, (G) uterine weights, (H) testicular weights, and (I) seminal vesicle weights. Bar heights are group means. Each white circle represents an individual mouse. Data were analyzed by two-tailed unpaired t tests with Welch's correlation. Bars with different letters differ significantly. Ovarian sections stained with hematoxylin and eosin (H&E) from (J) control and (K) cKO females. Scale bars: 200 μm.