Table 1.
Primers
| Genotyping primers | |
| Zeb1 F | CGTGATGGAGCCAGAATCTGACCCC |
| Zeb1 R | GCCCTGTCTTTCTCAGCAGTGTGG |
| Zeb1 R (recombinant) | GCCATCTCACCAGCCCTTACTGTGC |
| GRIC F | GGACATGTTCAGGGATCGCCAGGC |
| GRIC R | GCATAACCAGTGAAACAGCATTGCTG |
| Murine Lhb promoter amplification | |
| +5 promoter amplification rv | CGGAAGCTTTTGATACCTTCCCTACCTTGG |
| −435/+5 promoter amplification fw | CTAGGTACCCTTGAGTCCCCTGGAATCTG |
| −495/+5 promoter amplification fw | CAGGGTACCTCCAGGCGCAATTTACTGAT |
| −968/+5 promoter amplification fw | TTTGGTACCCCAATCCAGGCATCCTGATT |
| Lhb promoter mutagenesis | |
| −183E mLhb mut fw | GCTTCCAATGTCAGCTAAGCCCTGATGCCGAGGCCGAGTGTGAG |
| −183E mLhb mut rv | CTCACACTCGGCCTCGGCATCAGGGCTTAGCTGACATTGGAAGC |
| −30Z mLhb mut fw | ACCCCCACAACCCGTAGGCATAAAGCCAGGTGC |
| −30Z mLhb mut rw | GCACCTGGCTTTATGCCTACGGGTTGTGGGGGT |
| −30E mLhb mut fw | ACAACCCGCAGGTATAAAGCTGGGGACCCAAGGTAGGGAAGGTATC |
| −30E mLhb mut rw | GATACCTTCCCTACCTTGGGTCCCCAGCTTTATACCTGCGGGTTGT |
| qPCR primers | |
| Fshb F | GTGCGGGCTACTGCTACACT |
| Fshb R | CAGGCAATCTTACGGTCTCG |
| Gnrhr F | TTCGCTACCTCCTTTGTCGT |
| Gnrhr R | CACGGGTTTAGGAAAGCAAA |
| Lhb F | ACTGTGCCGGCCTGTCAACG |
| Lhb R | AGCAGCCGGCAGTACTCGGA |
| Rpl19 F | CGGGAATCCAAGAAGATTGA |
| Rpl19 R | TTCAGCTTGTGGATGTGCTC |
| Tgfrb3l F | CCTGACACCAGTGCCTTTGA |
| Tgfbr3l R | CTAGGGGACGGACGAGGTAT |
| Nr5a1 F | AGGAGTTCGTCTGTCTCAAGTTCCT |
| Nr5a1 R | ACAAGGTGTAATCCAACAGGGCAG |
| Zeb1 F | ATTCCCCAAGTGGCATATACA |
| Zeb1 R | GAGCTAGTGTCTTGTCTTTCATCC |