Fig. 6. Hydrodynamic size effects of i.v. administered PPS NP, dextran, and polystyrene tracer association with leukocytes within LNs. Number of (A) total cells, (B) T cells (CD3⁺B220⁻), (C) B cells (B220⁺CD3⁻CD11b⁻), (D) conventional dendritic cells [cDC] (CD11c⁺CD3⁻B220⁻), and (E) subcapsular sinus macrophages [SSM] (CD3⁻B220⁻CD11b⁺CD169⁺F4/80⁻CD11c^LO) in the lymph node associated with tracer 4 h post intravenous administration as a function of tracer hydrodynamic size. (F–J) Data in (A–E) normalized to quantity of tracer reaching the lymph node (% of injected amount) measured in tissue homogenate. *, **, ***, and **** indicate significant difference between tracer sizes by two way ANOVA. ^ indicates significant difference from all other tracer sizes except those also marked with ^ by two way ANOVA. #, ###, and #### indicate significant difference between materials for dextran and both polystyrene and PPS NP by two way ANOVA. n = 5 mice per group and results are representative of two independent experiments.