FIG. 3.

Requirement of the interaction of nectin-1α with afadin for efficient cell-cell spread, but not entry, of HSV-1. L, nectin-1α-L, and nectin-1α-ΔC-L cells were incubated at 37°C for 30 min with 10⁵ PFU of HSV-1(KOS)tk/well. At 6 h (A) or 2 days (B) after inoculation, the cells were fixed and stained with X-Gal. Upper panel, high magnification; lower panel, low magnification; bars, 200 μm. These results are representative of three independent experiments.