FIG. 4.

gD-binding activities of L and EL cells. (A) gD-binding activities. Confluent cells were subjected to a gD-binding assay. The binding was determined by ELISA. ○, L cells; ●, EL cells. (B) Western blotting. Each cell lysate of L and EL cells (20 μg of protein each) was subjected to SDS-PAGE (10% polyacrylamide gel), followed by Western blotting with the polyclonal anti-nectin-1α Ab2 and the monoclonal anti-afadin Ab. These results are representative of three independent experiments.