FIG. 7.

Inability of gD to inhibit cis dimerization of nectin-1α and -1α-ΔC. A single-cell suspension of nectin-1α-L or -1α-ΔC-L cells was incubated with various combinations of 2 μM gDl and 1 mM cross-linker bis-(sulfosuccinimidyl)-suberate. Each cell lysate was subjected to SDS-PAGE (8% polyacrylamide gel), followed by Western blotting using the polyclonal anti-nectin-1α Ab2. Arrowhead, dimer; arrow, monomer. These results are representative of three independent experiments.