Figure 3.

Mito‐transfer in naïve CD4⁺ T cells from old mice protects mice against pathogens & promotes protective T cell phenotypes in the lungs. A) 2.0 × 10⁶ naïve CD4⁺ T cells from young mice and old mice with or without mito‐transfer, where tail vein injected in TCR‐KO mice, that were the subsequently infected M.tb. A subset of mice were euthanized 30 days postinfection. Another subset of mice was treated with INH/RIF for an additional 30 days (up to 60 days postinfection). The absolute and percentage of various CD4⁺ T cell subsets in the lung of M.tb. infected mice were evaluated at both time points (30 and 60 days post‐infection). B) Absolute cell count of the digested lung from TCR‐KO mice infected with M.tb. The percents and absolute counts of C) CD3⁺CD4⁺ T cells, D) CD4⁺CD69⁺, E) CD4⁺CD25⁺, F) CD4⁺ CD69⁺ CD25⁺, G) CD4⁺ PD‐1⁺, H) CD4⁺CD27⁻ I) CD4⁺ PD‐1⁺, CD27⁻, J) CD4⁺ KLRG1⁺ K) CD4⁺ TIM3⁺ subsets isolated from TCR‐KO mice infected with M.tb. at 30 and 60 days post‐infection. p < 0.05= significant (^*) using one‐way‐ANOVA.