Figure 6.

Mito‐transfer phenotype and functionality recapitulated in aged human CD4⁺ T cells. A) Elderly CD4⁺ T cells with or without mito‐transfer were activated via surface cross‐linking (CD3/CD28). CD4⁺ T cells either received a single (single) or continuous (cont.) stimulation. The percentages of B) CD45RO⁺ CD62L⁺, C) CD45RO⁺ CD62L⁻, D) CD45RO⁻ CD62L⁻, E) CD45RO⁻ CD62L⁻, F) CD69⁺, G) PD‐1⁺, H) PD‐1⁺ LAG3⁺, I) PD1⁺ TIM3⁺, J) CD28⁺, K) CD27⁺, L) CD57⁺, M) KLRG1⁺, and N) β‐Gal‐FITC⁺, p16⁺ human CD4⁺ T cells at 7 days after initial activation with CD3/CD28/CD2. 10 biological replicates per group, p ≤ 0.05=^*, p ≤ 0.01=^**, p ≤ 0.001=^*** using paired Student's t‐test.