Figure 6.

G3BP1 is required for the oncogenic effect of DCAF7 on NPC progression. A) IB of G3BP1, DCAF7 and GAPDH in HONE1 and SUNE1 cells transfected with the indicated plasmids. B) Annexin V/PI staining and flow cytometric analysis of apoptosis in HONE1 and SUNE1 cells transfected with the indicated plasmids following cisplatin treatment (2.5 µg mL⁻¹) for 24 h. Mean (n = 3) ± s.d. One‐way ANOVA, ^** p < 0.01, ^*** p < 0.001. C) IB of Caspase3/9, cleaved Caspase3/9, G3BP1, DCAF7 and GAPDH in HONE1 and SUNE1 cells treated with cisplatin (10 µg mL⁻¹) for 24 h. D) Evaluation of apoptosis by a TUNEL assay in transfected NPC cells treated with cisplatin (10 µg mL⁻¹) for 24 h. The scale bars represent 20 µm. E) Transwell assays were conducted to assess cell migration and invasion, and representative images and quantitative results are presented. The scale bars represent 200 µm. Mean (n = 3) ± s.d. One‐way ANOVA, ^*** p < 0.001. F) IB of E‐cadherin, Vimentin, G3BP1, DCAF7 and GAPDH in HONE1 and SUNE1 cells transfected with the indicated plasmids. G) IF (with an anti‐E‐cadherin or anti‐Vimentin antibody) in HONE1 and SUNE1 cells transfected with the indicated plasmids. The scale bars represent 20 µm. The unprocessed images of the blots are shown in Figure S10 (Supporting Information).