Figure 2.

Effect of hybridization time on α and [SB'] for different levels of RNA abundance and differential expression. 2a) The variation in α with the hybridization time is shown for 8 different cases of abundance level and differential expression listed in Table 2. The parameters used are k_f = 10⁶ M^-1s^-1, k_f-bound = 10⁶ M^-1s^-1, k_b = 0 s^-1, initial genomic DNA concentration = 1 pM, bound strand concentration = 10⁶ pM, transport rate = 1 s^-1 2b) Change in concentration of SB', the intensity corresponding to the cDNA channel, with time for rare and intermediate species. □ S₀ = 0.1 pM, ○ S₀ = 1 pM, * S₀ = 2 pM