Table 1.
Nomenclature
| Symbol | Description |
| S | cDNA sample anti-sense strand |
| R | Genomic DNA anti-sense strand |
| R' | Genomic DNA sense strand |
| B | Bound (immobilized) anti-sense strand |
| B' | Bound (immobilized) sense strand |
| Subscript 0 | Initial concentration |
| SR' | Double strand formed in solution by hybridization of anti-sense cDNA strand and sense genomic DNA strand |
| RR' | Double strand formed in solution by hybridization of anti-sense and sense genomic DNA strands |
| SB' | Double strand formed on surface by hybridization of anti-sense cDNA strand and sense bound strands |
| RB' | Double strand formed on surface by hybridization of anti-sense genomic DNA strand and sense bound strands |
| R'B | Double strand formed on surface by hybridization of sense genomic DNA strand and anti-sense bound strands |
| h | Height of spot phase |
| r | Radius of spot phase |
| kb | Rate constant of backward reaction of hybridization |
| kf | Rate constant of forward reaction of hybridization between mobile species |
| kf-bound | Rate constant of forward reaction of hybridization between mobile and bound species |
| kt | Rate constant for transport between the two phases |
| γ | Ratio obtained from a hybridization assay using genomic DNA as a reference { = [SB']/([RB']+[R'B])} |
| α | Assay efficiency = (γ1/γ2)/(S10/S20), where 1 and 2 denote samples 1 and 2 |
| εS | Amount of S reacted with R' |
| εR | Amount of R reacted with R' |