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. 2024 Sep 23;29(1):2404794. doi: 10.1080/13510002.2024.2404794

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© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 6. — OPA1 overexpression relieved TRIM22 overexpression-induced apoptosis and mitochondrial dysfunction in HK-2 cells. Co-transduction of TRIM22 and OPA1 expression vector into HK-2 cells for 48 h. (A) CCK-8 was used to determine cell viability. (B, C) Flow cytometry was used to detect cell apoptosis, (D) mitochondrial ROS, and (E) MMP. (F) Biochemical assay was used to determine the ATP/ADP ratio. (G) Western blotting was used to detect OPA1 expression. ***P < 0.001 versus vector; ^###P < 0.001 versus TRIM22.