Fig 5.

Expression of isrBHDK and ssuCAB conferred the isethionate assimilation ability to R. sphaeroides. Plasmids expressing isrBHDK, ssuCAB (rcc02223-02225), and both gene clusters of isrBHDK and ssuCAB (isrBHDK +ssuCAB) were introduced into R. sphaeroides by conjugation, and growth of the transconjugants was monitored by OD₆₆₀ in the culture medium containing isethionate as the sole sulfur source under anaerobic photosynthetic conditions. The transconjugant carrying only the empty vector (emp) was the negative control.