TABLE 2.

List of genes conserved specifically in R. capsulatus identified by genome profiling analysis and specifically induced on the isethionate medium as the sole sulfur source (>100 Ise/Sul ratio) and the growth ability of the targeted disrupted mutants under anaerobic photosynthetic heterotrophic conditions on the isethionate medium

Locus tag (rcc)a	Annotationb	Ise/Sul ratioc	Growth on Ise cultured
02222	ntaA; nitrilotriacetate monooxygenase, component A	4968.0	+
02223	ABC transporter, permease protein (SsuC)	4662.3	–
02224	ABC transporter, substrate-binding protein (SsuA)	3422.2
02225	ABC transporter, ATP-binding protein (SsuB)	1259.4
02232	FAD-dependent oxidoreductase	3987.9	+
02234	Oxidoreductase/nitrogenase, component 1 (IsrK)	1748.9	–
02235	Oxidoreductase/nitrogenase, component 1 (IsrD)	1148.9
02236	nifH2; nitrogenase iron protein-2 (IsrH)	1819.7	–
02237	Radical SAM family protein (IsrB)	4787.6	–
02241	tauA; taurine ABC transporter, periplasmic taurine-binding protein TauA	931.6	+
02242	tauB; taurine ABC transporter, ATP-binding protein TauB	488.4
02243	tauC; taurine ABC transporter, permease protein TauC	687.1
02244	sufS2; cysteine desulfurase-2	1421.7	+
02245	Major membrane protein I	1292.6	+
02247	Rhodanese domain protein	1005.3	+

^a The five-digit number indicates the number following the locus tag of rcc.

^b Annotations in the GenBank database. The names of proteins proposed in this work are shown in parentheses.

^c The ratio of transcript levels of the gene in cells grown in media containing isethionate (Ise) and sulfate (Sul) as the sole sulfur sources.

^d Growth ability of the mutant lacking the gene(s) in the isethionate medium under anaerobic photosynthetic conditions.