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. 2024 Aug 27;9(9):e00504-24. doi: 10.1128/msphere.00504-24

Fig 1.

Stepwise illustration of the serial dilution process of HY in DPBS, contaminating surfaces, extracting with swabs, and performing a 10-fold dilution into tissue dilution solution.

Effective swabbing recovery of prions applied to laboratory surfaces. (A)Vertical dilution method surface contamination and swabbing methodology of contaminated laboratory surfaces. Created with BioRender.com. (B)RT-QuIC detection for glass slide surface-recovered HY prions. (C)RT-QuIC detection for stainless steel surface-recovered HY prions. (D)RT-QuIC detection for laboratory benchtop surface-recovered HY prions. Negative plate controls include blank (tissue dilution solution) and uninfected brain homogenate. A positive fluorescence threshold (illustrated by the red line) was determined to be at 2. The maxpoint ratio reported is the ratio of the maximum fluorescence to the initial fluorescence reading obtained by the plate reader. Each point represents the average MPR from one biological replicate (mean ± standard deviation).