Fig 7.
Nano RT-QuIC increases the sensitivity of RT-QuIC detection of surface-recovered HY. RT-QuIC and nano RT-QuIC detection of stainless steel surface-recovered HY dilutions from vertical dilution experiment. Negative plate controls include blank and uninfected brain homogenate dilution of 10−3 and 10−10. A positive fluorescence threshold (illustrated by the red line) was determined to be at an MPR of 2. The maxpoint ratio reported is the ratio of the maximum fluorescence to the initial fluorescence reading obtained by the plate reader. Each point represents the average MPR from one technical replicate (mean ± standard deviation).