TABLE 3.
Comparison of RT-QuIC and bioassay results of treated prion-contaminated glass slide surfaces
| RT-QuIC SD50/g of brain homogenate | RT-QuIC positive wells (10−3) | Incubation period (days)a | Attack rate | PrPSc present on western blot | |
|---|---|---|---|---|---|
| UN HA BH swab extract negative control | MPR< 2 | 0/4 | >300 | 0/5 | 0/5 |
| HY TME 10−4 positive control | 11.37 | 4/4 | 76 ± 3 | 5/5 | 5/5 |
| H2O-treated swab extract | 10.20 | 4/4 | 91 ± 7 | 5/5 | 5/5 |
| 70% ethanol-treated swab extract | 9.20 | 4/4 | 95 ± 4 | 5/5 | 5/5 |
| Bleach-treated swab extract | MPR< 2 | 0/4 | >300 | 0/5 | 0/5 |
a
Average ± SD.