Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Sep 24;25(1):2398801. doi: 10.1080/15384047.2024.2398801

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2024 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

PMC Copyright notice

Figure 1. — CD8HTM and CD28HTM GUCY2C CAR-T Production. (a) designs of CD8HTM and CD28HTM CARs. (b-i) CD8HTM and CD28HTM CAR constructs used were from 14-day manufacturing of CAR-T cells from n = 6 donors and matched comparisons between CARs. (b-c) expansion and viability of CD8HTM and CD28HTM CAR-T cell products. (d) CD8⁺ and CD4⁺ populations among CAR-transduced T cells. Flow cytometry plots shown. (e) Transduction efficiency (% GFP⁺) and construct expression (GFP MFI) for all 6 donors. (f-g) comparison of transduction efficiency (f) and construct expression (g) between CD8⁺ and CD4⁺ T cells for each CAR. (h-i) comparison of transduction efficiency (h) and construct expression (i) between CD8HTM and CD28HTM CARs for CD8⁺ and CD4⁺ T cells. All statistical comparisons are paired T-tests; symbols connected with a line represent a matched donor.