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. 2024 Sep 24;25(1):2398801. doi: 10.1080/15384047.2024.2398801

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© 2024 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 5. — Enhanced cytokine production and cytolytic potential by CD8HTM CAR compared to CD28HTM CAR. (a-h) CD8HTM and CD28HTM CAR-T cells produced from n = 3 donors were stimulated with plate-bound recombinant GUCY2C extracellular domain protein, and intracellular cytokines (a-f), and granzyme B (g-h) were quantified by flow cytometry. (a,d) comparison of CD8⁺ T cells (a) and CD4⁺ T cells (d) producing 0-3 cytokines between CD8HTM and CD28HTM CARs. * p = .048; *** p = .0005 (b) SPICE plots demonstrating the average number and type of cytokine expression by CD8HTM and CD28HTM CAR-T cells among CD8⁺ (b) and CD4⁺ (e) CAR-T cells. Individual cytokine expression comparison between CD8HTM and CD28HTM CAR-T cells among CD8⁺ (c) and CD4⁺ (f) CAR-T cells. Granzyme B comparison between CD8HTM and CD28HTM CAR-T cells among CD8⁺ (g) and CD4⁺ (h) CAR-T cells. Representative flow cytometry plots are shown. Statistical analyses for a and d were performed using two-way ANOVA adjusted for multiple comparisons. Error bars reflect the SEM. Statistical comparisons for c, f, g, and h are paired T-tests; symbols connected with a line represent a matched donor.