TABLE 2.

Formation of heterologous RV VLS

Co-expressiona		VLS formationc	Co-expressionb		VLS formation
NSP5 species	NSP2-HA species		NSP5-BAP species	NSP2-HA species
RVA	RVA	+	RVA	RVA	+
RVA	RVB	−	RVB	RVA	−
RVA	RVC	+, filamentous/globular	RVC	RVA	−
RVA	RVD	_	RVD	RVA	−, nuclear aggregates
RVA	RVF	−	RVF	RVA	−, nuclear inclusions
RVA	RVG	−	RVG	RVA	−, aggregates
RVA	RVH	−	RVH	RVA	−
RVA	RVI	+	RVI	RVA	−
RVA	RVJ	−	RVJ	RVA	−

^a Confirmed by co-immunostaining of NSP5 of RVA and NSP2 HA of species A–J using specific guinea pig pAb anti-NSP5 and mAb anti-HA, respectively.

^b Confirmed by co-immunostaining of NSP5-BAP of species A to J and NSP2-HA of RV species A using StAv and mAb anti-HA, respectively.

^c The result corresponds to the observation of three independent experiments.