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. 2024 Sep 18;137(18):jcs261895. doi: 10.1242/jcs.261895

Fig. 6.

Fig. 6.

Requirements for repression of HCP-3 chromatin localization by CSR-1. Representative images and quantification of the mean GFP::HCP-3 intensity of the metaphase plate in one-cell embryos with (A) knockdown of the RNA-dependent RNA polymerase of the CSR-1 RNAi pathway, EGO-1 [ego-1(RNAi)]; (B) isoform-specific knockdown of CSR-1A [csr-1a(RNAi)], compared to pan-CSR-1 depletion [csr-1a&b(RNAi)]; (C) knockdown of PRG-1, an Argonaute of the germline piRNA pathway, either alone [prg-1(RNAi)] or in combination with CSR-1 depletion; (D) knockdown of HRDE-1, an Argonaute of another germline RNAi pathway, either alone [hrde-1(RNAi)] or in combination with CSR-1 depletion; and (E) knockdown of endogenous CSR-1 in strains expressing transgenic, RNAi-resistant versions of either wild-type CSR-1 (CSR-1WT) or a slicer-inactive mutant (CSR-1SIN). Scale bars: 10 μm. GFP intensity is shown relative to the levels in the untreated controls. Data are presented as mean±95% confidence interval. The n values shown represent the number of one-cell embryos analyzed. **P<0.01; ***P<0.001; ****P<0.0001; ns, not significant (two-tailed unpaired t-test).