Fig. 7. Accelerated thymic involution in CypKO mice.
(A) UMAPs indicating decreased expression of pro-longevity factors Fgf21, Fgf7, and Igf1 in CypKO TECs. (B) Validation of thymic involution related gene expression. (C) Cell cycle phase scoring for representative WT and CypKO TECs. (D and E) Flow cytometry analysis of Ki67+ in mTEClo cells. (F) Total thymic cell counts from young to old mice showing accelerated involution in CypKO samples. (G) Frequency of mTEChi cells of total mTECs. (H) Frequency of cTECs of total TECs. All experiments were repeated at least twice with age-matched (male and female) mice. Each dot represents an individual mouse. Statistics: Unpaired parametric t tests. *P < 0.05; **P < 0.01; ****P < 0.0001.