Figure 7.

Therapeutic effect of Rtp4 knockdown on corneal HSV-1 infection in T2D mice. Knockdown of mouse Rtp4 by subconjunctival injection of siRNA was detected by quantitative PCR (A), Western blot (B), and immunofluorescence staining (C) in the cornea of T2D mice. (D) Rtp4 knockdown ameliorated the corneal phenotype in T2D mice at 5 Dpi. (E) Rtp4 knockdown decreased the corneal opacity scores in T2D mice at 5 Dpi. (F) H&E staining of corneal sections. (G) Rtp4 knockdown reduced the infiltration of immune cells (CD45+). (H) Staining for ICP4 and RTP4 of corneal sections. (I) Quantitative PCR analysis of HSV-1 viral genes were assessed in T2D mice at 5 Dpi. The m-Rpl5 served as an internal control. NC, negative control. ***P < 0.001.