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. 2005 May 23;102(22):7976–7981. doi: 10.1073/pnas.0503500102

Fig. 1.

Fig. 1.

Cellular uptake of molecular probes. (a) Internalization of Cyp-GRD in A549 cells incubated in 1 μM probe for 30 min at 37°C. (b) Inhibition of Cyp-GRD uptake by coincubation with 10 μM cyclo[GRDfV] and 1 μM Cyp-GRD at 37°C for 30 min. (c) Inhibition of Cyp-GRD uptake by coincubation with 10 μg/ml anti-β3 monoclonal antibody and 1 μM of Cyp-RGD at 37°C for 30 min. (d) Low-level inhibition of Cyp-GRD uptake by coincubation with 10 μg/ml anti-αv monoclonal antibody and 1 μM Cyp-RGD at 37°C for 30 min. (e) Cyp-GRD (red) and TROPO-1 (blue, nuclear stain) showing that probe is not localized in the nucleus. Images in b–d were normalized to the highest fluorescence intensity from a.