Figure 2.

Selection of SatIII transcripts. Total RNA from heat-shocked HeLa cells was annealed to a biotinylated oligonucleotide complementary to the transcribed strand of the SatIII sequences (Reverse/HS). The oligonucleotide was then captured on streptavidin-coated magnetic beads and unbound RNA washed away. As a control, we repeated the same procedure with 1) an oligonucleotide complementary to the reverse oligo (Forward/HS) and 2) with an oligonucleotide specific for a vector sequence (pBR322/HS). The reverse oligo was also hybridized to RNA purified from unstressed cells (Reverse/37°C). Bound and unbound fractions were then analyzed in slot blot by using the pHuR98 probe.