Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Ctnnb1 | GenBank | Gene ID: 12387 | |
| Gene (Homo sapiens) | CTNNB1 | GenBank | Gene ID: 1499 | |
| Genetic reagent (Mus musculus) | ieCtnnb1 knockout | This paper | N/A | Received from Beijing Biocytogen, China |
| Genetic reagent (Mus musculus) | ieCtnnb1-LacZ | This paper | N/A | Received from Shanghai Model Organisms Center Inc, China |
| Genetic reagent (Homo sapiens) | ieCTNNB1-LacZ | This paper | N/A | Received from Shanghai Model Organisms Center Inc, China |
| Strain, strain background (Mus musculus) | BAT-Gal | The Jackson Laboratory | JAX#005317; RRID:IMSR_JAX:005317 | |
| Strain, strain background (Mus musculus) | Lgr5-EGFP-IRES-CreERT2 | The Jackson Laboratory | JAX#008875; RRID:IMSR_NM-KI-200154 |
|
| Strain, strain background (Mus musculus) | ApcMin/+ | The Jackson Laboratory | JAX#002020; RRID:MGI:3834882 | |
| Cell line (Homo sapiens) | HEK293T | ATCC | CRL-1573; RRID:CVCL_0045 | |
| Cell line (Homo sapiens) | HCT116 | China Center for Type Culture Collection | GDC0625; RRID:CVCL_0291 | |
| Cell line (Homo sapiens) | HCT-15 | China Center for Type Culture Collection | GDC0250; RRID:CVCL_0292 | |
| Cell line (Homo sapiens) | HeLa | China Center for Type Culture Collection | GDC0009; RRID:CVCL_0030 | |
| Transfected construct (Homo sapiens) | pHR-SFFV-dCas9-BFP-KRAB | Addgene | RRID:Addgene #46911 | |
| Transfected construct (Homo sapiens) | lenti sgRNA (MS2)_zeo backbone vector | Addgene | RRID:Addgene #61427 | |
| Transfected construct (Homo sapiens) | dCAS9-VP64_GFP | Addgene | RRID:Addgene #61422 | |
| Transfected construct (Homo sapiens) | lenti MS2-P65-HSF1_Hygro | Addgene | RRID:Addgene #61426 | |
| Antibody | Anti-α-defensin5 (Rabbit monoclonal) | Abcam | CAT#ab180515; RRID:AB_2923196 |
IHC (1:500) |
| Antibody | Anti-Ki67 (Rabbit polyclonal) | Abcam | CAT#ab15580; RRID:AB_443209 |
IHC (1:500) |
| Antibody | Anti-β-catenin (Rabbit monoclonal) | Cell Signaling Technology | CAT#8480T; RRID:AB_11127855 |
IHC (1:100) WB (1:1000) |
| Antibody | Anti-HNF4α (Rabbit monoclonal) |
Abcam | CAT#ab181604; RRID:AB_2890918 |
ChIP (1 µg/ml) |
| Antibody | Anti-CREB1(S133) (Rabbit monoclonal) | Abcam | CAT#ab32096; RRID:AB_731734 |
ChIP (1 µg/ml) |
| Antibody | Anti-Muc2 (Rabbit polyclonal) |
Thermo Fisher | CAT#PA5-21329; RRID:AB_11153058 |
IHC (1:500) |
| Antibody | Anti-EpCAM FITC (Mouse monoclonal) | eBioScience | CAT#347197; RRID:AB_400261 |
FACS (1:200) |
| Recombinant DNA reagent | pGL3-promoter-ieCtnnb1 | This paper | N/A | Obtained from Zhou Lab of Wuhan University |
| Recombinant DNA reagent | pGL3-promoer-ieCTNNB1 Region1 | This paper | N/A | Obtained from Zhou Lab of Wuhan University |
| Recombinant DNA reagent | pLKO.1-shRNAs | This paper | N/A | Obtained from Zhou Lab of Wuhan University |
| Sequence-based reagent | TF-shRNAs | This paper | PCR primers | See Supplementary file 1 |
| Sequence-based reagent | ieCtnnb1/ ieCTNNB1 ChIP-qPCR primers |
This paper | ChIP-qPCR primers | See Supplementary file 1 |
| Sequence-based reagent | Genotyping primers | This paper | PCR primers | See Supplementary file 1 |
| Commercial assay or kit | Dual-Luciferase Reporter Assay System | Promega | CAT#E1910 | |
| Commercial assay or kit | Alcian Blue PAS Stain Kit | Abcam | CAT#ab245876 | |
| Commercial assay or kit | BeyoClick EdU Cell Promotion Kit | Beyotime | CAT#C0075S | |
| Software | Fiji/ImageJ | https://fiji.sc | v2.0.0; RRID:SCR_003070 | |
| Software | GraphPad Prism | https://www.graphpad.com | v8.0; RRID:SCR_002798 | |
| Other | DAPI | BD Pharmingen | CAT#564907 | (1 µg/ml) |