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. 2005 Jun;16(6):2694–2703. doi: 10.1091/mbc.E05-01-0036

Figure 6.

Figure 6.

Inhibition of PMN transepithelial migration by soluble JAML and CAR recombinant proteins. As detailed in Materials and Methods, soluble CAR and JAML recombinant proteins (20 μg/ml) were added to the upper chamber of inverted T84 cell monolayers followed by the addition of PMN. Migration in the presence of JAM-A fusion protein, GST or Fc only (25 μg/ml) and no fusion protein (HBSS) served as controls. PMN transmigration was initiated by adding fMLP (1 μM) to the lower chamber and migration into the lower chamber quantified by MPO assay. (A) PMN migration after 1 h; (B) a time course PMN transepithelial migration over 3 h in the presence or absence of the specified fusion proteins. Data represent mean ± SE of three independent experiments performed in duplicate (p < 0.01).